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poly a enrichment with oligo dt 25 magnetic beads  (New England Biolabs)


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    Structured Review

    New England Biolabs poly a enrichment with oligo dt 25 magnetic beads
    Poly A Enrichment With Oligo Dt 25 Magnetic Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 979 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/poly+a+enrichment+with+oligo+dt+25+magnetic+beads/Oligo+d(T)25+Magnetic+Beads/pm38630600-82-12-19
    Average 98 stars, based on 979 article reviews
    poly a enrichment with oligo dt 25 magnetic beads - by Bioz Stars, 2026-10
    98/100 stars

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    Magnetic Beads:

    Article Title: Signatures of mRNA Alternative Polyadenylation in Arabidopsis Leaf Development
    Article Snippet: Following isolation, the DNA in RNA samples was removed using DNase I (Takara) and was purified in an RNA clean column, and then 2 μg of the total RNA was used to construct PAT-seq libraries as described previously ( ). .. Briefly, the total RNA was fragmented at 94°C for 4 min, followed by poly(A) enrichment with oligo (dT) 25 magnetic beads (New England Biolabs). .. Next, the fragments of poly(A) enriched RNA was reverse transcribed using barcoded oligo (dT) primers with the SMARTScribe enzyme (Clontech) for 2 h, and then 5’ adapter for template switching was added after 2 h. The cDNAs were purified using AMPURE XP beads (Beckman), and then were amplified with PCR for 18 cycles with Phire II (Thermo Fisher Scientific).

    Article Title: Growth conditions trigger genotype-specific metabolic responses that affect the nutritional quality of kale cultivars.
    Article Snippet: Briefly, a total amount of 1 μg RNA per sample was used to construct RNA-seq libraries using NEBNext UltraTM RNA Library Prep Kit for Illumina (New England D ow nloaded from https://academ ic.oup.com /jxb/advance-article/doi/10.1093/jxb/erae169/7648744 by guest on 22 April 2024 Ac ce pte d M an us cri pt Biolabs, USA). .. The total RNA was fragmented at 65°C for 5 min, followed by poly(A) enrichment with oligo (dT)25 magnetic beads (New England Biolabs, USA). .. Double stranded cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (New England Biolabs, USA), adenylated, and ligated with NEBNext adaptor. cDNA fragments of 150~200 bp in length were purified using AMPure X Pbeads (Beckman Coulter, Beverly, USA).



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